Restriction Enzymes
New England Biolabs, Inc. PvuI-HF – 2500 units
A high fidelity restriction endonuclease that recognizes the sequence CG_AT^CG. High Fidelity (HF) Restriction Enzymes have 100% activity in rCutSmart Buffer; single-buffer simplicity means more straightforward and streamlined sample processing. HF enzymes also exhibit dramatically reduced star activity. HF enzymes are all Time-Saver qualified and can therefore cut substrate DNA in 5-15 with the flexibility to digest overnight without degradation to DNA. Engineered with performance in mind, HF restriction enzymes are fully active under a broader range of conditions, minimizing off-target products, while offering flexibility in experimental design. NEB extensively performs quality controls on all standard and high-fidelity (HF) restriction enzymes. Examples of nuclease contamination studies for some of our HF restriction enzymes are shown below.
New England Biolabs, Inc. Tsp45I – 200 units
A restriction endonuclease that recognizes the sequence ^GTSAC_.
New England Biolabs, Inc. AvaII – 10000 units
A restriction endonuclease that recognizes the sequence G^GWC_C.
New England Biolabs, Inc. HpaII – 10000 units
A restriction endonuclease that recognizes the sequence C^CG_G.
New England Biolabs, Inc. FokI – 5000 units
A restriction endonuclease that recognizes the sequence GGATGNNNNNNNNN^NNNN_.
Electron Microscopy Sciences TEM Specimen Supports Molybdenum 1000 µm
We have extended our range of TEM grid materials to include four types, which are now available in Molybdenum. The new products are manufactured using a process known as chemical ʽmilling' (etching) instead of the more familiar technique of ʽelectroforming' (deposition) that is used in the manufacture of copper, nickel and gold products. Molybdenum is used principally in applications where it's high temperature, hardness, expansion of coefficient and corrosion resistance characteristics are considered important. The material which is used has a purity of 99.9%.
Hole: 1000 µm
Thickness: 50 µm
New England Biolabs, Inc. BstEII-HF – 10000 units
A high fidelity restriction endonuclease that recognizes the sequence G^GTNACC. High Fidelity (HF) Restriction Enzymes have 100% activity in rCutSmart Buffer; single-buffer simplicity means more straightforward and streamlined sample processing. HF enzymes also exhibit dramatically reduced star activity. HF enzymes are all Time-Saver qualified and can therefore cut substrate DNA in 5-15 with the flexibility to digest overnight without degradation to DNA. Engineered with performance in mind, HF restriction enzymes are fully active under a broader range of conditions, minimizing off-target products, while offering flexibility in experimental design. NEB extensively performs quality controls on all standard and high-fidelity (HF) restriction enzymes. Examples of nuclease contamination studies for some of our HF restriction enzymes are shown below.
Apexbio Technology LLC Dpn I(Synonyms: DpnI, DpnI Restriction Enzyme, Dam methylation-sensitive restriction enzyme DpnI), 1000U.
Dpn I is a restriction endonuclease sensitive to Dam methylation specifically recognizing and cleaving the adenine N6-methylated sequence GA/TC within DNA Cleavage is dependent on adenine methylation status mammalian CpG methylation overlapping Dpn I sites blocks cleavage and methylation by Dcm is not recognized by this enzyme It is routinely used for the rapid digestion of plasmid DNA PCR fragments and genomic DNA and finds broad utility in genetic typing molecular cloning Southern blotting SNP genotyping and restriction fragment length polymorphism (RFLP) analyses
New England Biolabs, Inc. SfiI – 3000 units
A restriction endonuclease that recognizes the sequence GGCCN_NNN^NGGCC.
New England Biolabs, Inc. SmaI – 10000 units
A restriction endonuclease that recognizes the sequence CCC_^GGG.
Sigma Aldrich Fine Chemicals Biosciences β-Glucuronidase from Escherichia coli | CAS 9027-70-9 | 1,000,000 Units
β-glucuronidase from E. coli | Purity: ≥90% | Mol Wt: 68 kDa | CAS No: 9027-70-9 | Unit: 1,000,000 Units
Cayman Chemical BAM-22P8-22humnmouse1mg
A neuropeptide and MRGPRX1 agonist; induces calcium mobilization in HEK293S cells expressing human MRGPRX1 or SNSR3 (EC50s = 14 and 28 nM, respectively); inhibits voltage-induced calcium currents in isolated rat neurons expressing human MRGPRX1 (EC50 = 0.6 µM); intrathecal administration decreases the tail-flick latency in pertussis toxin-stimulated rats and increases the tail-flick latency in U-73122-stimulated rats, indicating changes in pain sensitivity, at 30 nmol/animal; increases scratching behavior in wild-type mice and enhances bile-duct ligation-induced scratching behavior in a mouse model of cholestasis
New England Biolabs, Inc. Endonuclease V – 250 units
Endonuclease V is a repair enzyme found in E. coli that recognizes deoxyinosine, a deamination product of deoxyadenosine in DNA. Endonuclease V, often called deoxyinosine 3 endonuclease, recognizes DNA containing deoxyinosines (paired or not) on double-stranded DNA, single-stranded DNA with deoxyinosines and to a lesser degree, DNA containing abasic sites (ap) or urea, base mismatches, insertion/deletion mismatches, hairpin or unpaired loops, flaps and pseudo-Y structures. It is believed that Endonuclease V needs another protein to repair the DNA, as it does not remove the deoxyinosine or the damaged bases. Endonuclease V cleaves the second phosphodiester bonds 3' to the mismatch of deoxyinosine, leaving a nick with 3'-hydroxyl and 5'-phosphate.
New England Biolabs, Inc. NotI-HF® – 2500 units
A high fidelity restriction endonuclease that recognizes the sequence GC^GGCC_GC. High Fidelity (HF) Restriction Enzymes have 100% activity in rCutSmart Buffer; single-buffer simplicity means more straightforward and streamlined sample processing. HF enzymes also exhibit dramatically reduced star activity. HF enzymes are all Time-Saver qualified and can therefore cut substrate DNA in 5-15 minutes with the flexibility to digest overnight without degradation to DNA. Engineered with performance in mind, HF restriction enzymes are fully active under a broader range of conditions, minimizing off-target products, while offering flexibility in experimental design. NEB extensively performs quality controls on all standard and high-fidelity (HF) restriction enzymes. Examples of nuclease contamination studies for some of our HF restriction enzymes are shown below.
- Reduced star activity
- Supplied with 1 vial of Gel Loading Dye, Purple (6X)